This provides a quasi-3D rendering of the specimen with depth perception. This is used when the specimen is not thin enough to allow electrons through. In scanning electron microscopes, electrons reflecting off the specimen provide information about the surface of the specimen. The image is identified and labeled with the help of preexisting knowledge about the permeability of particular substances to electrons. The parts that allow the electrons to pass through appear bright. The parts that absorb the electrons prevent their passage to the screen, and thus appear dark. The specimen lies above the fluorescent screen, and hence, the electrons that make a mark on the screen are the ones able to pass through the specimen. On the other hand, electron microscopes form images based on the selective absorption of electrons by various parts of the specimen.
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